British Journal of Oral and Maxillofacial Surgery
Volume 46, Issue 2 , Pages 110-113, March 2008

Processed bovine dentine as a bone substitute

  • Keyvan Moharamzadeh

      Affiliations

    • School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 114 2717924; fax: +44 114 2665326.
    • ,
  • Christine Freeman

      Affiliations

    • Sheffield Teaching Hospitals NHS Trust, Charles Clifford Dental Hospital, Wellesley Road, Sheffield S10 2SZ, UK
    • Tel.: +44 114 2717849; fax: +44 114 2717863.
    • ,
  • Keith Blackwood

      Affiliations

    • School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK

Accepted 25 July 2007. published online 26 September 2007.

Abstract 

Objectives

Different forms of allogenic dentine have been studied for their potential use as bone substitutes. We report a new method for processing bovine dentine that results in a sterile bioactive material for repair and regeneration of bone.

Methods

Extracted bovine dentine was processed mechanically and chemically with inorganic and organic solvents, and sterilised. The in vitro biocompatibility on human gingival fibroblasts was assessed by the Alamar Blue assay and the in vivo biocompatibility evaluated by implantation of the processed dentine into rats’ femurs.

Results

The dentine showed excellent biocompatibility in vitro, stimulated formation of new bone and was completely incorporated into the new bone in vivo.

Significance

Processed bovine dentine has the potential to be used as a suitable substitute in bone repair and regeneration.

Keywords: Dentine, Bone regeneration, Biocompatibility, Alamar Blue assay

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PII: S0266-4356(07)00383-X

doi:10.1016/j.bjoms.2007.07.209

British Journal of Oral and Maxillofacial Surgery
Volume 46, Issue 2 , Pages 110-113, March 2008

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