Vitalisation of tubular coral scaffolds with cell sheets for regeneration of long bones: a preliminary study in nude mice

Gao, Zhan; Chen, Fulin; Zhang, Junrui; He, Lisheng; Cheng, Xiaobin; Ma, Qin; Mao, Tianqiu

doi:10.1016/j.bjoms.2008.07.199

« Previous Next »British Journal of Oral and Maxillofacial Surgery
Volume 47, Issue 2 , Pages 116-122, March 2009

Vitalisation of tubular coral scaffolds with cell sheets for regeneration of long bones: a preliminary study in nude mice

Zhan Gao
- Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146^#, Xi’an 710032, P.R. China
Fulin Chen
- RegeMed Lab of Tissue Engineering, Faculty of Life Science, Northwest University, Tai Bai Bei Road 229#, Xi’an 710069, P.R. China
Junrui Zhang
- Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146^#, Xi’an 710032, P.R. China
Lisheng He
- Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146^#, Xi’an 710032, P.R. China
Xiaobin Cheng
- Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146^#, Xi’an 710032, P.R. China
Qin Ma
- Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146^#, Xi’an 710032, P.R. China
Tianqiu Mao
- Department of Oral &Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Chang Le Xi Road 146^#, Xi’an 710032, P.R. China
- Corresponding author. Tel.: +86 29 84776102; fax: +86 29 83223047.

Accepted 23 July 2008. published online 07 November 2008.

Abstract

In this study, cell sheets comprising multilayered living bone marrow stromal cells and extracellular matrix were assembled with tubular coral scaffolds for long bone regeneration. Cell sheet with visible mineralized nodules was harvested and wrapped against tubular coral scaffolds with 5mm diameter and 1.5mm wall thickness. New bone formation was investigated by CT scan and histological observation 8 and 12 weeks after implantation of cell sheet/scaffold. The results showed that cortical bone formed within the constructs for both groups. New bone composed 25.75% of the graft in 8 weeks group, compared to that of 40.01% in 12 weeks group. Histological examination showed that new bone formation was in the manner of endochondral osteogenesis, with woven bone matrix subsequently maturing into fully mineralized compact bone. These findings demonstrated that osteogenic cell sheet could vitalize tubulate coral scaffolds to regenerate bone graft with similar shape and structure to native bone.