Nystatin — Resistance of candida albicans isolates from two cases of oral candidiasis

      This paper is only available as a PDF. To read, Please Download here.


      Two cases of oral candidiasis are described which failed to respond to nystatin therapy when used in combination with triamcinolone acetonide. The isolates of C. albicans obtained from the patients after treatment showed high in vitro resistance to nystatin when tested in combination with triamcinolone acetonide. Triamcinolone acetonide was detected in the saliva of both patients after treatment. Addition of this saliva to the isolates of C. albicans obtained after treatment was found to confer nystatin resistance. Both patients were treated with miconazole nitrate and a mycological and clinical cure was obtained in one of the cases.


        • Athar M.A.
        • Winner H.I.
        The development of resistance by Candida species to polyene antibiotics in vitro.
        Journal of Medical Microbiology. 1971; 4: 505
        • Aufdemorte T.B.
        • McPherson M.A.
        Refractory oral candidosis.
        Oral Surgery. 1978; 46: 776
        • Beveridge G.W.
        • Fairburn E.
        • Finn O.A.
        • Scott O.L.S.
        • Stewart T.W.
        • Summerly R.
        A comparison of nystatin cream with nystatin/triamcinolone acetonide in the treatment of candidal inflammation of the flexors.
        Current Medical Research Opinion. 1977; 8: 584
        • Bodenhoff J.
        Development of strains of genus Candida and genus Torulopsis resistant to antimycotics.
        Acta Pathologica Microbiologica Scandinavica. 1969; 75: 622
        • Dolan C.T.
        A practical approach to the identification of yeast-like organisms.
        American Journal of Clinical Pathology. 1971; 55: 580
        • Hamilton-Miller J.M.T.
        Non-emergence of polyene-resistant yeast: an hypothesis.
        Microbios. 1974; 10A: 91
      1. Holt, R. J. Antifungal drugs. In Reeves, D. S., Phillips, I., Williams, J. D. & Wise, R. (editors): Laboratory methods in antimicrobial chemotherapy, pp. 249–253. Edinburgh, Churchill-Livingstone.

        • Kirschbaum J.
        • Poet R.
        • Bush K.
        • Petrie G.
        High-performance liquid chromatography of the topical anti-inflammatory steroid halcinomide.
        Journal of Chromatography. 1980; 190: 481
        • Lampen J.O.
        • Arnow P.M.
        • Safferman R.S.
        Mechanism of protection by sterols against polyene antibiotics.
        Journal of Bacteriology. 1959; 80: 200
        • Lightbrown J.W.
        • Sulitzeanu D.
        The assay of penicillin in blood serum using Sarcina lutea.
        Bulletin of the World Health Organisation. 1957; 17: 553
        • Moss M.S.
        • Rylance H.J.
        The detection and identification of synthetic steroids in horse urine.
        Journal of Pharmacy and Pharmacology. 1966; 18: 13
        • Nobre G.M.
        • Pereira A.
        • Coias R.
        • Cordeiro J.
        In vitro development of resistance to nystatin by Candida albicans and Torulopsis glabrata.
        Journal of General Microbiology. 1980; 118: 263
        • Odds F.C.
        Candida and Candidosis.
        in: Leicester University Press, Leicester1979: 228-231
        • Pingleton W.W.
        • Bone R.C.
        • Kerby G.R.
        • Ruth W.E.
        Oropharyngeal candidiasis in patients treated with triamcinolone acetonide aerosol.
        Journal of Allergy and Clinical Immunology. 1977; 60: 254
        • Ritchie G.M.
        • Fletcher A.M.
        • Main D.M.G.
        • Prophet A.S.
        The etiology, exfoliative cytology, and treatment of denture stomatitis.
        Journal of Prosthetic Dentistry. 1969; 22: 185
        • Tygart G.N.
        • Surachno S.
        • De Groot W.P.
        • Schellekens P.T.
        A case of chronic oropharyngoesophageal candidiasis with immunological deficiency: successful treatment with miconazole.
        Gastroenterology. 1970; 72: 536
        • Zygmunt W.A.
        • Tavormina P.A.
        Steroid interference with antifungal activity of polyene antibiotics.
        Applied Microbiology. 1966; 14: 865