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Nitric oxide synthase expression is downregulated in basal cell carcinoma of the head and neck

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      Abstract

      The small molecule nitric oxide (NO) has many actions, most of which are poorly understood. Recently, NO and related compounds have been implicated in skin damage caused by ultraviolet light although their exact role is not clear. We undertook an immuno histochemical study to assess the expression of type II NO synthase (NOS2) and type III (NOS3) in basal cell carcinomas (BCCs) of the head and neck. In all 48 cases studied, NOS2 was found in the basal cell layer of the skin at the tumour margin but it w as significantly reduced in the tumour epithelial cells (P=0.001). NOS3 was localized to the endothelium of the blood vessels in both skin and tumour in all cases, and it was not seen in the tumour epithelial cells. The results suggest that expression of NOS is down-regulated in basal cell carcinomas.
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      References

      References

        • Beckman JS
        • Koppenol WH
        Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and the ugly.
        Am J Physiol. 1996; 271: 1424-1437
        • Gaston B
        • Drazen JM
        • Loscalzo J
        • Stamler JS
        The biology of nitrogen oxides in the airways.
        Am J Resp Crit Care Med. 1994; 149: 538-551
        • Bruch-Gerharz D
        • Ruzicka T
        • Kolb-Bachofen V
        Nitric oxide and its implications in skin homeostasis and disease – a review.
        Arch Dermatol Res. 1998; 290: 643-651
        • Villiotou V
        • Deliconstantinos G
        Nitric oxide, peroxynitrite and nitroso-compounds formation by ultraviolet A (UVA) irradiated human squamous cell carcinoma: potential role of nitric oxide in cancer prognosis.
        Anticancer Res. 1995; 15: 931-934
        • Suschek CV
        • Bruch-Gerharz D
        • Berendgi D
        Nitric oxide fully protects against UVA-induced apoptosis in tight correlation with Bcl-2 up-regulation.
        J Biol Chem. 1999; 274: 6130-6137
        • Grossman D
        • Leffell DJ
        The molecular basis of non melanoma skin cancer: new understanding.
        Arch Dermatol. 1997; 133: 1263-1270
        • Foerstermann U
        • Kleinert H
        Nitric oxide synthase: expression and expressional control of the three isoforms.
        Naunyn-Schmeidebergs Arch Pharmacol. 1995; 352: 351-364
        • Shimizu Y
        • Sakai M
        • Umemura Y
        • Ueda H
        Immunohistochemical localisation of nitric oxide synthase in normal human skin: expression of endothelial-type and inducible-type nitric oxide synthase in keratinocytes.
        J Dermatol. 1997; 24: 80-87
        • Ahmad B
        • Van Den Oord JJ
        Expression of the neuronal isoform of nitric oxide synthase (nNOS) and its inhibitor, protein inhibitor of nNOS, in pigment cell lesions of the skin.
        Br J Dermatol. 1999; 141: 12-19
        • Schmidt HHHW
        • Pollock JS
        • Nakane M
        • Förstermann U
        • Murad F
        Ca2+calmodulin-regulated nitric oxide synthases.
        Cell Calcium. 1992; 13: 427-434
        • Albina JE
        • Reichner JS
        Role of nitric oxide in mediation of macrophage cytotoxicity and apoptosis.
        Cancer Metastasis Rev. 1998; 17: 39-53
        • Thomsen LL
        • Miles DW
        Role of nitric oxide in tumour progression: lessons from human tumours.
        Cancer Metastasis Rev. 1998; 17: 107-118
        • Brennan PA
        • Umar T
        • Buckley J
        • Zaki GA
        • Spedding AV
        • Langdon JD
        Nitric oxide synthase expression in pleomorphic adenoma of the parotid.
        Br J Oral Maxillofac Surg. 2000; 38: 338-342
        • Tschugguel W
        • Pustelnik T
        • Lass H
        Inducible nitric oxide synthase (iNOS) expression may predict distant metastasis in human melanoma.
        Br J Cancer. 1999; 79: 1609-1612
        • Heck DE
        • Laskin DI
        • Gardner CR
        • Laskin JD
        Epidermal growth factor suppresses nitric oxide and hydrogen peroxide mediation by keratinocytes: potential role for nitric oxide in the regulation of wound healing.
        J Biol Chem. 1992; 267: 21277-21280
        • Krischel V
        • Bruch-Gerharz D
        • Suschek C
        • Kroncke KD
        • Ruzicka T
        • Kolb-Bachofen V
        Biphasic effect of exogenous nitric oxide on proliferation and differentiation in skin derived keratinocytes but not fibroblasts.
        J Invest Dermatol. 1998; 111: 286-291
        • Gallo O
        • Masini E
        • Morbidelli L
        Role of nitric oxide in angiogenesis and tumour progression in head and neck cancer.
        J Natl Cancer Inst. 1998; 90: 587-596
      1. Brennan, PA, Palacios-Callender, M, Zaki, GA, Spedding, AV, Langdon, JD, Type II nitric oxide synthase expression correlates with lymph node status in oral squamous cell carcinoma, Journal of Oral Pathology and Medicine

        • Fukumura D
        • Yuan F
        • Endo M
        • Jain RK
        Role of nitric oxide in tumour microcirculation: blood flow, vascular permeability and leukocyte-endothelial interactions.
        Am J Pathol. 1997; 150: 713-725