Abstract
The aim of this study was to examine the effectiveness of covering wounds to the tongue
with a polyglycolic acid (PGA) sheet and fibrin glue. Eighteen mature male Japanese
white rabbits had a unilateral glossectomy involving an area 10 mm × 10 mm × 2 mm. After glossectomy the tongues were covered with PGA sheets 8 mm × 8 mm in size and fibrin glue (mucosal defect covered with fibrin glue and polyglycolic
acid sheet = MCFP) 1 week after the operation (n = 3), after 2 weeks (n = 3), and after 4 weeks (n = 3). In control groups, after 1, 2, and 4 weeks (n = 3 in each group), the partially resected tongues were closed with absorbable sutures
(polyglactin 910). One week (experimental and control groups 1), 2 weeks (experimental
and control groups 2) and 4 weeks (experimental and control groups 3) after operation
the tongues were harvested and stained for microscopic examination. Histological examination
showed that the covered wound surface had not epithelialised and the basal layer had
yet to form in experimental group 1, but had formed in experimental group 2. However,
in control group 1, epithelialisation of the sutured wound had begun. Immunohistochemical
examination showed that, in experimental group 1, the non-uniform epithelial layer
of the covered wound surface expressed cytokeratin AE1/AE3, and the epithelial and
connective tissue layers stained strongly for FGF-2. Similar results were obtained
in experimental group 2, whereas in experimental group 3, FGF-2 was expressed only
in the connective tissue layer, and epithelialisation was complete. However, in control
group 1, AE1/AE3 was expressed in the epithelial layer, and FGF was expressed in the
connective tissue layer beneath the basal layer. In control groups 2 and 3, AE1/AE3
and FGF-2 were expressed in patterns similar to those in experimental groups 2 and
3. We suggest that this method is useful and the operation is simple. However, further
testing of the method is needed and it should be widely used clinically before it
is recommended.
Keywords
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Article info
Publication history
Published online: August 08, 2011
Accepted:
July 16,
2011
Identification
Copyright
© 2011 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.
