Abstract
The present study examined the possible use of autologous human plasma (AHP) to improve
the in vitro culture for cytogenetics of benign and malignant oral tumor cells.
The effect of AHP on the growth of a variety of benign and malignant head and neck
tumor cells was studied by inverted microscope and cytogenetic analysis. Minced tumor
biopsies of cases of hemangioma (HM), lipoma (LP), central giant cell granuloma (GC),
pleomorphic adenoma (PA), schwannoma (SW), oral squamous cell carcinoma (SCC), Ewing
sarcoma (ES), Merkel cell carcinoma (MCC) and adenoid cystic carcinoma (ACC), were
cultured in medium supplemented with either AHP, allogeneic pooled human plasma (PHP),
or fetal calf serum (FCS).
More fibroblasts were seen in the FCS supplemented cultures, while in HP or PHP culture
medium, more epithelial-like tumor cells were noted. The karyotypes of HM, LP, GC
and PA were normal in all 3 different mediums.
Cytogenetic analysis of SW and SCC revealed random numerical changes in all cultures.
However, in AHP cultures a clone bearing translocation was found in SW t(2;13). In
the SCC cultures one tumor had t(12;14) and the other t(3;21) translocations. Complex
karyotype was found in all kinds of cultures in ES, MCC and ACC.
AHP by itself does not cause chromosomal aberrations but may improve the ability to
find chromosomal aberrations in some tumors over medium containing FCS. The spectrum
of oral tumors where AHP can improve the cytogenetic analyses should be further studied
on a greater number of tumors.
Keywords
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Article info
Publication history
Published online: January 09, 2012
Accepted:
December 13,
2011
Identification
Copyright
© 2011 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.
