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Potential of autogenous or fresh-frozen allogeneic bone block grafts for bone remodelling: a histological, histometrical, and immunohistochemical analysis in rabbits

      Abstract

      Our aim was to compare the wound healing of autogenous bone grafts with that of fresh-frozen allogeneic block bone in rabbits. We used 25 animals. One was killed before the experiment to provide the allogeneic bone, and the remainder were killed at four time points (n = 6 in each group). On histometrical analysis there was a significant difference between the two groups only at 45 days and between 15 and 45 days in the intergroup analysis. However, there was significantly more revascularisation (p < 0.05), resorption (p < 0.05), and bony replacement (p < 0.05) in the autogenous group in the immunohistochemical analysis. In later periods, the autogenous bone was replaced by newly-formed bone in all samples, whereas it was always possible to find regions of devitalised bone in the fresh-frozen allogeneic bone grafts. Autogenous grafts were completely replaced whereas, in the fresh- frozen allogeneic grafts, we found acellular tissue that had been incorporated into the receptor bed interface during the later evaluation times.

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      References

        • Pinholt E.M.
        • Haanaes H.R.
        • Donath K.
        • et al.
        Titanium implant insertion into dog alveolar ridges augmented by allogenic material.
        Clin Oral Implants Res. 1994; 5: 213-219
        • Lumetti S.
        • Galli C.
        • Manfredi E.
        • et al.
        Correlation between density and resorption of fresh-frozen and autogenous bone grafts.
        Biomed Res Int. 2014; 2014: 508328
        • McAllister D.R.
        • Joyce M.J.
        • Mann B.J.
        • et al.
        Allograft update: the current status of tissue regulation, procurement, processing, and sterilization.
        Am J Sports Med. 2007; 35: 2148-2158
        • Buck B.E.
        • Malinin T.I.
        Human bone and tissue allografts. Preparation and safety.
        Clin Orthop Relat Res. 1994; 303: 8-17
        • Ellis III, E.
        • Sinn D.P.
        Use of homologous bone in maxillofacial surgery.
        J Oral Maxillofac Surg. 1993; 51: 1181-1193
        • Becker W.
        • Becker B.E.
        • Caffesse R.
        A comparison of demineralized freeze-dried bone and autologous bone to induce bone formation in human extraction sockets.
        J Periodontol. 1994; 65 (Erratum: J Periodontol 1995; 66:309): 1128-1133
        • Petrungaro P.S.
        • Amar S.
        Localized ridge augmentation with allogenic block grafts prior to implant placement: case reports and histologic evaluations.
        Implant Dent. 2005; 14: 139-148
        • Piattelli A.
        • Scarano A.
        • Piattelli M.
        Microscopic and histochemical evaluation of demineralized freeze-dried bone allograft in association with implant placement: a case report.
        Int J Periodontics Restorative Dent. 1998; 18: 355-361
        • Ullmark G.
        • Obrant K.J.
        Histology of impacted bone-graft incorporation.
        J Arthroplasty. 2002; 17: 150-157
        • Pimentel A.C.
        • Napimoga M.H.
        • Manzi M.R.
        • et al.
        Reconstruction of the edentulous mandible with fresh frozen bone grafts and implants: a 4-year report of a prospective clinical study.
        Cell Tissue Bank. 2014; 15: 1-6
        • Ramalho-Ferreira G.
        • Faverani L.P.
        • Momesso G.A.
        • et al.
        Effect of antiresorptive drugs in the alveolar bone healing. A histometric and immunohistochemical study in ovariectomized rats.
        Clin Oral Investig. 2016; : 1-10
        • Queiroz T.P.
        • Hochuli-Vieira E.
        • Gabrielli M.A.
        • et al.
        Use of bovine bone graft and bone membrane in defects surgically created in the cranial vault of rabbits. Histologic comparative analysis.
        Int J Oral Maxillofac Implants. 2006; 21: 29-35
        • Marx R.E.
        • Garg A.K.
        Bone structure, metabolism, and physiology: its impact on dental implantology.
        Implant Dent. 1998; 7: 267-276
        • Rogers G.F.
        • Greene A.K.
        Autogenous bone graft: basic science and clinical implications.
        J Craniofac Surg. 2012; 23: 323-327
        • Spin-Neto R.
        • Landazuri Del Barrio R.A.
        • Pereira L.A.
        • et al.
        Clinical similarities and histological diversity comparing fresh frozen onlay bone blocks allografts and autografts in human maxillary reconstruction.
        Clin Implant Dent Relat Res. 2013; 15: 490-497
        • Davy D.T.
        Biomechanical issues in bone transplantation.
        Orthop Clin N Am. 1999; 30: 553-563
        • Mizutani A.
        • Fujita T.
        • Watanabe S.
        • et al.
        Experiments on antigenicity and osteogenicity in allotransplanted cancellous bone.
        Int Orthop. 1990; 14: 243-248
        • Cypher T.J.
        • Grossman J.P.
        Biological principles of bone graft healing.
        J Foot Ankle Surg. 1996; 35: 413-417
        • Contar C.M.
        • Sarot J.R.
        • da Costa M.B.
        • et al.
        Fresh-frozen bone allografts in maxillary ridge augmentation: histologic analysis.
        J Oral Implantol. 2011; 37: 223-231
        • Spin-Neto R.
        • Stavropoulos A.
        • Coletti F.L.
        • et al.
        Graft incorporation and implant osseointegration following the use of autologous and fresh-frozen allogeneic block bone grafts for lateral ridge augmentation.
        Clin Oral Implants Res. 2014; 25: 226-233
        • Pereira E.
        • Messias A.
        • Dias R.
        • et al.
        Horizontal resorption of fresh-frozen corticocancellous bone blocks in the reconstruction of the atrophic maxilla at 5 months.
        Clin Implant Dent Relat Res. 2015; 17: e444-e458
        • Deluiz D.
        • Oliveira L.
        • Fletcher P.
        • et al.
        Fresh-frozen bone allografts in maxillary alveolar augmentation: analysis of the complications, adverse outcomes and implant survival.
        J Periodontol. 2016; 87: 1261-1267