Abstract
The aim of the study was to evaluate the association between genetic polymorphisms
in human epidermal growth factor (EGF) (rs4444903) and transforming growth factor
β1 – (TGF-β1) (rs1800470) with facial measurements in patients with dentofacial deformities.
A total of 144 adult patients with dentofacial deformities were included. Facial linear
and angular measurements were traced in lateral cephalometric radiographs used Dolphin
2D software. Cells from oral mucosa were collected for DNA to be extracted. The polymorphisms
were genotyped using real-time polymerase chain reaction (PCR). Probabilites of less
than 0.05 were accepted as significant. The rs4444903 heterozygous patients had a
decrease in the mandibular length (p = 0.043) and the length of the mandibular base (p = 0.008), and homozygous A patients also had a reduction in the length of the mandibular
base (p = 0.013) compared with homozygous G patients. Patients AG had an increase in measurement
of the anterior facial height (p = 0.032) and in ANS-Me distance (p = 0.022) when compared with homozygous A. To the rs1800470, heterozygous patients had
an increase in the length of the mandibular base (p = 0.043) when compared with homozygous A. Heterozygous AG patients had an increase in
angular measurements in TGF-β1 polymorphism for the upper gonial angle, when compared
with the homozygous AA (p = 0.032). Genetic polymorphisms in EGF and TGF-β1 are associated with facial measurements
in a Brazilian population of patients with dentofacial deformities.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to British Journal of Oral and Maxillofacial SurgeryAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Growth and development of dentofacial complex influenced by genetic and environmental factors using monozygotic twins.J Contemp Dent Pract. 2017; 18: 754-758
- Epidermal growth factor, from gene organization to bedside.Semin Cell Dev Biol. 2014; 28: 2-11
- The functional EGF+61 polymorphism and nonsyndromic oral clefts susceptibility in a Brazilian population.J Appl Oral Sci. 2015; 23: 390-396
- Spatial distribution of epidermal growth factor transcripts and effects of exogenous epidermal growth factor on the pattern of the mouse dental lamina.Arch Oral Biol. 1995; 40: 137-143
- Computational modelling of Smad-mediated negative feedback and crosstalk in the TGF-β superfamily network.J R Soc Interface. 2013; 1020130363
- The mechanism of TGF-β signaling during palate development.Oral Dis. 2011; 17: 733-744
- Enhancement of toluidine blue staining by transforming growth factor-beta, insulin-like growth factor and growth hormone in the temporomandibular joint of aged mice.Cells Tissues Organs. 2000; 167: 121-129
- Use of buccal epithelial cells for PCR amplification of large DNA fragments.J Forensic Odontostomatol. 2000; 18: 6-9
- A simple and cost-effective protocol for DNA isolation from buccal epithelial cells.Braz Dent J. 2007; 18: 148-152
- Growth hormone receptor gene variant and mandibular height in the normal Japanese population.Am J Orthod Dentofacial Orthop. 2001; 119: 650-653
- The growth hormone receptor gene is associated with mandibular height in a Chinese population.J Dent Res. 2005; 84: 1052-1056
- The P561T polymorphism of the growth hormone receptor gene has an inhibitory effect on mandibular growth in young children.Eur J Orthod. 2009; 31: 536-541
- Genetic polymorphism in RANK is associated with mandibular size.J Orthod. 2018; 45: 157-162
- Genetic variants in ACTN3 and MYO1H are associated with sagittal and vertical craniofacial skeletal patterns.Arch Oral Biol. 2019; 97: 85-90
- New three-dimensional cephalometric analyses among adults with a skeletal Class I pattern and normal occlusion.Korean J Orthod. 2013; 43: 62-73
- Three-dimensional cephalometric norms of Chinese adults in Hong Kong with balanced facial profile.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011; 112: e56-e73
- 3D CBCT McNamara’s cephalometric analysis in an adult southern Chinese population.Int J Oral Maxillofac Surg. 2011; 40: 920-925
- Evaluation of craniofacial proportions: A pilot study.Dent Res J (Isfahan). 2015; 12: 260-264
- Mandibular sexual dimorphism analysis in CBCT scans.J Forensic Leg Med. 2016; 38: 106-110
- Sexual dimorphism of the mandible in a contemporary Chinese Han population.Forensic Sci Int. 2015; 255: 9-15
- Transforming growth factor-beta signalling in extraembryonic mesoderm is required for yolk sac vasculogenesis in mice.Development. 1999; 126: 3473-3483
- Divergence of epidermal growth factor – transforming growth factor beta signaling in embryonic orofacial tissue.In Vitro Cell Dev Biol Anim. 2003; 39: 257-261
- TGF beta 1 regulation of collagen metabolism by embryonic palate mesenchymal cells.J Exp Zool. 1994; 270: 189-201
- Type II collagen and TGF-betas in developing and aging porcine mandibular condylar cartilage: immunohistochemical studies.Cell Tissue Res. 1997; 289: 119-124
Article info
Publication history
Published online: January 13, 2020
Identification
Copyright
© 2019 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
