Abstract
Squamous cell carcinoma (SCC) of the tongue is the most common oral cancer and is
prone to develop regional lymph nodes and distant metastases. Reliable and stable
therapeutic targets can improve the curative effect and reduce toxic side effects
caused by traditional treatments such as surgery, radiotherapy, and chemotherapy.
We have analysed three sets of series of functional gene expression of SCC of the
tongue from gene expression omnibus (GEO) datasets, and 154 common differentially
expressed genes (DEG) between SCC of the tongue and the corresponding normal tissues
were screened. Further bioinformatics research that was based on the data from the
Cancer genome atlas, Gene ontology, and the Kyoto encyclopaedia of genes and genomes indicated that the increased expression of follistatin might be correlated with a
poor prognosis in these patients. By assay of colony formation, reverse transcription
polymerase chain reaction (RT-PCR), western blotting, immunohistochemical staining,
and lentivirus transfection, we confirmed that downregulation of follistatin inhibited
the proliferation of SCC cells in the tongue.
Graphical abstract
Graphical Abstract
Keywords
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Article info
Publication history
Published online: February 27, 2020
Accepted:
January 29,
2020
Identification
Copyright
© 2020 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
