Abstract
The aim of this study was to compare the changes in the oral flora of Sprague-Dawley
rats before intraperitoneal injection of zoledronic acid, 6 weeks and 12 weeks after
injection. Clinically, some antibiotics effectively treat patients with medicine-related
osteonecrosis of the jaws (MRONJ), but the effect of broad-spectrum antibiotics for
osteomyelitis of the jaw is not obvious. We therefore speculated that MRONJ may have
some dominant bacteria. We used 12 healthy rats for the experiment. One rat was used
for haematoxylin and eosin staining, three were used for gene analysis, three for
signal molecule research, and five for 16SrDNA high-pass sequencing to compare the
changes of flora before intraperitoneal injection of zoledronic acid, and 6 and 12
weeks after injection once every three days. Alpha and beta analysis was used for
sequencing data. Analysis of the flora showed that the alpha diversity of the bacteria
of rats injected with zoledronic acid was significantly higher than it was before
injection (p < 0.05). At the phylum level, Bacteroidetes at 6 and 12 weeks of injection were significantly higher than those before injection
(p < 0.05). At the genus level, the proportions of Novophingobium, Dubosiella, Mannheimia, Prevotella, Brevundimonas, and Bacteroides were higher than they were before injection (p < 0.05). The proportions of Lactobacillus, Mannheimia, Brevundimonas, Bacteroides, Roseovarius, Salegentibacter, Marinobacter, and Granulicatella in rats injected for 12 weeks were higher than those before injection (p < 0.05). Zoledronic acid can change the structure of oral flora in SD rats, in which
Bacteroides increased and Actinomycetes decreased.
Keywords
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Article Info
Publication History
Published online: April 16, 2022
Accepted:
April 1,
2022
Publication stage
In Press Corrected ProofFootnotes
☆This study was supported by the Tianjin Municipal University Science and Technology Development Fund Project (grant 2019KJ174).
Identification
Copyright
© 2022 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
