Abstract
Mechanical stress induces a variety of biochemical and morphological reactions in
bone cell biology. This study aimed to investigate appropriate pressures of osteogenesis
on the biological responses of 3-dimensional cultured human mandibular fracture haematoma-derived
cells by compressive loading. Six patients with mandibular fractures who underwent
open reduction and internal fixation were included in the study. During the operation,
fracture haematomas that formed fibrin clots were manually removed before irrigation.
First, pressures were applied to human mandibular fracture haematoma-derived cell-seeded
collagen sponges. The sponges were subjected to mechanical compression using loading
equipment applied at no compression, 0.5, or 1 mm. Compressive loading was applied
to the samples prior to compression for 0, 6, 12, or 24 hours. Collagen sponge samples
were collected for quantification of mRNA using several parameters including alkaline
phosphatase (ALP), osteopontin (OPN), osterix (OSX), runt-related gene 2 (RUNX2),
protein level, and immunocytochemistry (anti-sclerostin). Among these the 0.5 mm compression
group compared with the control and 1.0 mm compression groups upregulated mRNA expression
of OPN and OSX after 24 hours. Additionally, compared with the control group, a significantly
higher OSX gene expression was observed in both the 0.5 mm and 1.0 mm groups after
6, 12, and 24 hours of compression (p < 0.05). However, no significant differences were observed regarding ALP and RUNX2
expression. These results indicated increased stimulation of osteogenesis of the mandibular
fracture-line gap in the 0.5 mm compression group compared with the control and 1.0 mm
compression groups.
Keywords
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Article Info
Publication History
Published online: June 10, 2022
Accepted:
June 2,
2022
Received in revised form:
May 31,
2022
Received:
December 20,
2021
Identification
Copyright
© 2022 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
