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Effect of Compression on Mandibular Fracture Hematoma-derived Cell

      Abstract

      Mechanical stress induces a variety of biochemical and morphological reactions in bone cell biology. This study aimed to investigate appropriate pressures of osteogenesis on the biological responses of three-dimensional cultured human mandibular fracture hematoma-derived cells by compressive loading. A total of six patients with mandibular fractures who underwent open reduction internal fixation were included in the study. During the operation, fracture hematomas that formed fibrin clots were manually removed before irrigation. First, pressures were applied to human mandibular fracture hematoma-derived cell-seeded collagen sponges. The collagen sponges were subjected to mechanical compression using the loading equipment applied at non-compression, 0.5, or 1 mm. Compressive loading was applied to the samples prior to compression at 0, 6, 12, or 24h. Collagen sponge samples were collected for quantification of mRNA using several parameters including: alkaline phosphatase(ALP), osteopontin (OPN), osterix (OSX), runt-related gene 2 (Runx2), protein level and immunocytochemistry (anti-sclerostin). Among these parameters, 0.5 mm compression group compared to the control group and the 1.0 mm compression group upregulated the mRNA expression of OPN and OSX after 24h. Additionally, a significantly higher OSX gene expression was observed in both the 0.5 mm and 1.0 mm groups after 6, 12, and 24 h of compression compared to the control group (p<0.05). However, no significant differences were observed regarding the ALP and RUNX2 expression. These results indicated an increased stimulation of osteogenesis of the mandibular fracture line gap in the 0.5 mm compressed group compared to the control group and the 1.0 mm compression group.

      Keywords

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