Abstract
Porcine mandibular defect models are commonly used for the preclinical evaluation
of reconstruction techniques. Existing studies vary in technique, complexity, and
postoperative outcomes. The procedures are complex and often described without sufficient
detail. We describe in detail a simple and reproducible method for creating a critical-size
mandibular defect in a porcine model. Seven hemimandibular critical size defects were
created in five male Yorkshire-Landrace pigs, three with unilateral defects and two
with bilateral defects. A transverse incision was made over the mandibular body. Periosteum
was incised and elevated to expose the mandibular body and a critical-size defect
of 30 × 20 mm created using an oscillating saw. The implant was inserted and fixed
with a titanium reconstruction plate and bicortical locking screws, and the wound
closed in layers with resorbable sutures. Intraoral contamination was avoided. Dentition
was retained and the mental nerve and its branches preserved. The marginal mandibular
nerve was not encountered during dissection. All pigs retained normal masticatory
function, and there were no cases of infection, wound breakdown, haematoma, salivary
leak, or implant-related complications. The procedure can be performed bilaterally
on both hemimandibles without affecting load-bearing function. All pigs survived until
the end point of three months. Postoperative computed tomographic scans and histology
showed new bone formation, and a three-point bend test showed the restoration of biomechanical
strength. Straight-segment mandibulectomy is a simple and reproducible method for
the creation of critical-size mandibular defects in a porcine model, simulating a
load-bearing situation.
Keywords
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Article info
Publication history
Published online: November 03, 2022
Accepted:
November 1,
2022
Identification
Copyright
© 2022 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
