Abstract
Cleft palate is a common maxillofacial congenital malformation, and its mechanism
still has not been fully illustrated. Recently, lipid metabolic defects have been
observed in cleft palate. Patatin-like phospholipase domain-containing 2 (Pnpla2)
is an important lipolytic gene. However, its effect on the formation of cleft palate
remains unknown. In this research, we explored the expression of Pnpla2 in the palatal
shelves of control mice. We also studied mice with cleft palates induced by retinoic
acid and its effect on the embryonic palatal mesenchyme (EPM) cells phenotype. We
found that Pnpla2 was expressed in the palatal shelves of both the cleft palate and
control mice. Pnpla2 expression was lower in cleft palate mice than in the control
mice. Experiments with EPM cells showed that knockdown of Pnpla2 inhibited cell proliferation
and migration. In conclusion, Pnpla2 is linked to palatal development. We have indicated
that low expression of Pnpla2 affects palatogenesis by inhibiting the proliferation
and migration of EPM cells.
Keywords
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Article info
Publication history
Published online: February 22, 2023
Accepted:
February 13,
2023
Received:
December 20,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2023 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
